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The amino-terminal BRCT motifs of Esc4 bind to Slx4
| LexA hybrid | GAD hybrid | Number of isolates |
| LexA-Esc4 (1–480) | GAD-Slx4 (166–748) | 1 |
| LexA-Esc4 (1–480) | GAD-Slx4 (194–748) | 2 |
| LexA-Esc4 (1–480) | GAD-Slx4 (260–748) | 1 |
| LexA-Esc4 (1–480) | GAD-Slx4 (280–748) | 4 |
| LexA-Esc4 (1–480) | GAD-Slx4 (368–748) | 3 |
| LexA-Esc4 (1–480) | GAD-Slx4 (383–748) | 2 |
