Table 1.

HPV primers used in PCR amplification of the HPV 16 genes.

Primer	DNA sequence	Gene target	Nucleotide positions in NCBI no NC_001526
L1_Sfor	5-GCCGTCGACATGTCTCTTTGGCTGCCTAGTAGGGCCA-3'	Late protein L1	5637–5663
L1_Srev	5'- GCCGTCGACGATTTGTAGTAAAAATTTGCGTCC-3'	Late protein L1	7027–7050
L2_Bfor	5'-GCGCGGATCCATGcgacacaaacgttctgcaa -3'	Late protein L2	4235–4256
L2_Hrev	5'-GCGCAAGCTTCTAGGCAGCCAAAGAGACATC -3'	Late protein L2	5636–5656
E6_Bfor	5'-GCGCGGATCCATGCACCAAAAGAGAACTGC3'	Early protein E6	83–102
E6_Hrev	5'-GCGCAAGCTTTTACAGCTGGGTTTCTCTACGTG -3'	Early protein E6	537–559
E4_Bfor	5'-GCGCGGATCCATGTATTATGTCCTACATCTGTGTTT -3'	Early protein E4	3332–3357
E4_Hrev	5'-GCGCAAGCTTCTATGGGTGTAGTGTTACTATTA-3'	Early protein E4	3597–3619

The start and stop codons are bolded while the restriction sites (Sal I, Bam HI, and Hind III) are underlined