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Oligonucleotides used in this study
| Primer | Sequence (5′ to 3′)a |
|---|---|
| S83W-T | GCTGCGCCATGCGGACGATCGTGTCA TAGACCGCCCAGTCACCATGGGGAT GGTATTTACCGATTACGTCA |
| gyrA-QF | GACCTTGCGAGAGAAATTACAC |
| gyrA-QR | GATGTTGGTTGCCATACCTACG |
| gyrA-IF1 | TGCTGGTGAACGGTTCTTCC |
| gyrA-IR1 | GTACGACGGGTCACCACTTC |
| gyrA-IF2 | CATCGCGGCGTTTGTTCGTC |
| gyrA-IR2 | CTTAACGTAGCCCTGGTGAG |
| gyrA-IF3 | TCACCCAGGAAGATGTGGTC |
| gyrA-IR3 | GCCACGAGGCACGATCAGAG |
| gyrA-IF4 | GTATTCGCTTAGGTGAAGGC |
| gyrA-IR4 | CCAGACTTTGCAGCCTGGAC |
A point mutation is boldfaced.
