TABLE 1.
Sequences of primers used for detection of various genes from pIP1204
| Gene | Primer name | Sequence (5′ → 3′) | Position | GenBank accession no. or reference |
|---|---|---|---|---|
| blaTEM | tem1F | TCGGGGAAATGTGCGCG | 4458-4433 | X67018 |
| tem1R | TGCTTAATCAGTGAGGCACC | 3477-3496 | ||
| ISEcp1 | ecpF | AAAAATGATTGAAAGGTGGT | 603-622 | AF454633 |
| blaCTX-M | P2D | CAGCGCTTTTGCCGTCTAAG | NAa | 13 |
| intI1 | intI1F | ACATGTGATGGCGACGCACGA | 1542-1562 | M95287 |
| intI1R | ATTTCTGTCCTGGCTGGCGA | 2110-2091 | ||
| sul1 | sulF1 | ATGGTGACGGTGTTCGGCAT | 4321-4340 | U12441 |
| sulR | CTAGGCATGATCTAACCCTC | 5160-5141 | ||
| aac3-IIa | aac3F | ATGCATACGCGGAAGGCAAT | 105-124 | L22613 |
| aac3R | CTAACCTGAAGGCTCGCAA | 965-947 | ||
| ant3"9 | aad2F | ATGAGGGTAGCGGTGACCAT | 1918-1937 | AF227505 |
| aad2R | TCATTTACCAACTGACTTGA | 2709-2690 | ||
| dhfrXII | dhfr12F | GCCAATCGGGTTATTGGCAA | 151-170 | AF335108 |
| dhfr12R | TGGGAAGAAGGCGTCACCCTC | 507-487 | ||
| armA | metF | CAAATGGATAAGAATGATGTT | 1975-1995 | This work and AY220558 |
| metR | TTATTTCTGAAATCCACT | 2751-2734 |
a
NA, not applicable.