FIG. 1.
Restriction fragment analysis of vanB long PCR amplicons. BspHI/DraI-digested vanB long PCR amplicons were analyzed by agarose gel electrophoresis. Lanes: 1 and 6, 1-kb ladder (Invitrogen Corporation, Carlsbad, Calif.); 2, vanB1 strain V583 with RFLP-1; 3, vanB2 strain 4-G10 with RFLP-2; 4, vanB2 strain 4-C11 with a 1,504-bp insertion in vanSB2 leading to replacement of the 969-bp fragment with restriction fragments of 1,327, 614, and 532 bp (RFLP-2#); 5, vanB2 isolate 5-F9 with RFLP-2. Molecular sizes shown to the left of the gel (in base pairs) refer to the 1-kb ladder.