TABLE 1.

Origin and one-letter amino acid sequences for flatfish cationic antimicrobial peptides synthesized and tested for activities^a

Origin	Amino acid sequence	Code	No. of residues	Mol wt	Charge
Winter flounder (1)	GKGRWLERIGKAGGIIIGGALDHL-NH2	NRC-01b,c	24	2,487	+3.5
Winter flounder (1a)	WLRRIGKGVKIIGGAALDHL-NH2	NRC-02c	20	2,172	+4.5
Winter flounder (1a-1)	GRRKRKWLRRIGKGVKIIGGAALDHL-NH2	NRC-03c	26	2,954	+9.5
Winter flounder (2) 2.1	GWGSFFKKAAHVGKHVGKAALTHYL-NH2	NRC-04b,c	25	2,711	+6.5
Winter flounder (3)	FLGALIKGAIHGGRFIHGMIQNHH-NH2	NRC-05c	24	2,625	+5.0
Winter flounder (4) 1.1	GWGSIFKHGRHAAKHIGHAAVNHYL-NH2	NRC-06c	25	2,764	+6.5
Yellowtail flounder YT2	RWGKWFKKATHVGKHVGKAALTAYL-NH2	NRC-07	25	2,854	+8.0
Winter flounder X	RSTEDIIKSISGGGFLNAMNA-NH2	NRC-08b,c	21	2,180	+2.0
Winter flounder Y	FFRLLFHGVHHGGGYLNAA-NH2	NRC-09b,c	19	2,112	+3.5
Winter flounder Z	FFRLLFHGVHHVGKIKPRA-NH2	NRC-10b,c	19	2,260	+6.5
American plaice AP1	GWKSVFRKAKKVGKTVGGLALDHYL-NH2	NRC-11	25	2,759	+6.5
American plaice AP2	GWKKWFNRAKKVGKTVGGLAVDHYL-NH2	NRC-12	25	2,859	+6.5
American plaice AP3	GWRTLLKKAEVKTVGKLALKHYL-NH2	NRC-13	23	2,653	+6.5
Witch flounder GcSc4C5	AGWGSIFKHIFKAGKFIHGAIQAHND-NH2	NRC-14	26	2,851	+4.5
Witch flounder GcSc4B7	GFWGKLFKLGLHGIGLLHLHL-NH2	NRC-15	21	2,356	+4.5
Witch flounder GC3.8-td	GWKKWLRKGAKHLGQAAIK-NH2	NRC-16	19	2,175	+7.5
Witch flounder GC3.8	GWKKWLRKGAKHLGQAAIKGLAS	NRC-17	23	2,505	+6.5
Witch flounder GC3.2	GWKKWFTKGERLSQRHFA	NRC-18	18	2,262	+4.5
Halibut Hb26	FLGLLFHGVHHVGKWIHGLIHGHH-NH2	NRC-19	24	2,749	+5.5
Halibut Hb18	GFLGILFHGVHHGRKKALHMNSERRS	NRC-20	26	2,985	+6.0

^aIn order to estimate the net charge, K and R were assumed to have values of +1, H was assumed to have a value of of +, and D and E of were assumed to have values of −1; C-terminal amidation was counted as an additional +1.

^bPeptide activities were published previously as described in Materials and Methods.

^cClone sequences were published previously, as described in Materials and Methods.

^dTruncated form of GC3.8.