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. 2000 Jul 5;97(14):7721–7726. doi: 10.1073/pnas.97.14.7721

Figure 1.

Figure 1

Purification of TelN protein. Aliquots of extracts and pooled peak fractions were resolved with an SDS/PAGE (15%) and stained with Serva Blue R after electrophoresis. Expression of telN in SCS1(pJD101) was chemically induced in the presence of 1 mM isopropyl β-d-thiogalactopyranoside. Cells were lysed by either SDS or Brij-58/lysozyme. The amount of total protein loaded onto the gel is given in parentheses. Lanes a and b, SDS extract of noninduced and isopropyl β-d-thiogalactopyranoside-induced cells, respectively (31 μg, 34 μg); lane c, Brij-58/lysozyme extract of induced cells (51 μg); lane d, fraction I (46 μg); lane e, fraction II (9.2 μg); lane f, fraction III (14.5 μg); lane g, fraction IV (4.1 μg); lane h, fraction V (3.9 μg); lane i, molecular mass standards.