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. 2000 Jul 5;97(14):7721–7726. doi: 10.1073/pnas.97.14.7721

Figure 5.

Figure 5

Optimization of reaction conditions for TelN enzymatic activity. The reactions were performed as described in Materials and Methods, with 0.3 pmol of TelN protein and 0.15 pmol of pJD104 per assay. (A) Effect of NaCl (■), KCl (♦), and potassium glutamate (▴). (B) Effect of pH. (C) Effect of temperature. (D) Time dependency of pJD104 (■) [pBR329 (♦)] processing by TelN.