Figure 6.

Product analysis of TelN enzymatic activity. Products of the reaction outlined in the legend to Fig. 4 were analyzed on a 0.7% and a 1% agarose gel under nondenaturing and alkaline conditions, respectively. One microgram of DNA per lane was applied. Electrophoresis was carried out under nondenaturing conditions [45 mM Tris-borate, pH 8.0/1 mM EDTA (10 V/cm for 90 min)] and under alkaline conditions [1 mM EDTA/50 mM NaOH (6 V/cm for 4.5 h)] (16, 17). Gels were stained with ethidium bromide and a FluorImager scan is shown. Lanes a and a′, size marker (1-kb DNA ladder); lanes b and b′, pJD104 after reaction with AatII and TelN; lanes c and c′, pJD104 after reaction with MscI and TelN, both shown in Fig. 3C, III.