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Daptomycin dissipates membrane potential in S. aureus, as measured by flow cytometry. S. aureus cell samples were incubated for 0 (control), 15 (D15), 30 (D30), or 60 (D60) min with 5 μg of daptomycin/ml or for 10 min with 25 μg of nisin (N10)/ml. (A) Individual traces. (B) Changes in membrane potential (as measured by the ratio of fluorescence emission intensities at 610 and 530 nm) and cell viability, both expressed as percentages of the untreated control.
