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Hemolytic activities for halocidin congeners were tested against human erythrocytes. Triton X-100 (1%) was used as the control for 100% hemolysis, and 0.01% acetic acid was used as the peptide-free control. P18 and melittin were used as standard peptides. The percent hemolysis was calculated with the following equation: hemolysis (%) = (A540 of sample − A540 of peptide-free control)/(A540 of 100% control − A540 of peptide-free control) × 100.
