Skip to main content
. 2000 Jul 5;97(14):7748–7753. doi: 10.1073/pnas.97.14.7748

Figure 4.

Figure 4

BRG-1 expression restores RB-mediated arrest in BRG-1 deficient cells. (A) SW13 cells were cotransfected with the −608Luc, CMV-β-gal, and vector, PSM-RB or PSM-RB + BRG-1. Samples were then processed for luciferase activity and normalized to β-gal activity. (B) SW13 cells were cotransfected with either vector, BRG-1, or PSM-RB + BRG-1 along with pBabe-PURO. Transfected cells were selected with puromycin, and protein lysates were resolved by SDS/PAGE and subjected to immunoblotting for cyclin A, Cdk2 and Cdk4. (C) SW13 cells were cotransfected with either vector, p16ink4a, PSM-RB, BRG-1, p16ink4a + BRG-1, or PSM-RB + BRG-1 and H2B-GFP expression plasmids. Cells were labeled with BrdUrd, and the percent of transfected cells incorporating BrdUrd is shown.