. Author manuscript; available in PMC: 2006 Nov 29.

Published in final edited form as: Chem Res Toxicol. 2006 Nov;19(11):1426–1434. doi: 10.1021/tx060142s

Table 1.

Affinity Isolation of Cytosolic Glutathione Transferase.

A. PB-Control^a	Volume (mL)	Protein (mg)	Activity (U)^b
Load	10.5	140	34.8
FT + Wash	25	121	8.5
NaCl Wash	14	2.0	0.3
Hex-SG	13.5	7.7	6.2^c
B. [¹⁴C]-BB^a	Volume (mL)	Protein (mg)	¹⁴C (nCi)
Load	11.8	204	3431
FT + Wash	49	197	3141
NaCl Wash	17	3.7	21
Hex-SG	8.6	9.8	95

Refer to Experimental Procedures for details of column loading and elution.

One unit is 1 μmol product/min/mg protein at room temperature.

May be underestimated because of inhibition by residual S-hexyl-glutathione.