Table 3.
Apparent Masses of HPLC-Separated GST Subunits from PB-Control Rats as Determined by ESI-MS.
| HPLC peak | GST subunit assigneda | Observed mass (Da) | Theoretical mass (Da) | Δ (Da)b |
|---|---|---|---|---|
| 1 | GSTM1 | 25786 | 25782 | 4 |
| 2 | GSTM1 | 25787 | 25782 | 4 |
| 3 | GSTM2 | 25611c | 25571 | 40d |
| 4 | GSTA3 | 25193 | 25188 | 5 |
| 5 | GSTA1 | 25522 | 25522e | 0 |
| 6 | GSTA2 | 25475 | 25470e | 5 |
a
See text for basis of subunit assignment.
b
Observed mass minus theoretical mass.
c
Determined by MALDI-TOF MS.
d
This isoform may be acetylated on its N-terminus, based on the mass difference.
e
Includes 42 Da for reported N-terminal acetylation on this isoform.