Table 4.
GST Subunit Identification by Peptide Mass Mapping.
| HPLC peak | GST subunit assigned | Number of peptide matchesa | Percent sequence coverage | MOWSE score |
|---|---|---|---|---|
| BB-Treated | ||||
| 1 | GSTM1 | 20 | 57 | 4.59 E+05 |
| 3 | GSTM2 | 30 | 72 | 2.26 E+10 |
| 4 | GSTA3 | 19 | 53 | 4.63 E+02 |
| 5 | GSTA2b | 23 | 52 | 4.92 E+05 |
| 5 | GSTA1b | 25 | 50 | 1.43 E+05 |
| 6 | GSTA2 | 20 | 56 | 1.03 E+04 |
| PB-Control | ||||
| 1 | GSTM1 | 6 | 30 | 4.59 E+01 |
| 2 | GSTM1 | 24 | 66 | 1.61 E+07 |
a
Peptide masses determined by MALDI-TOF MS.
b
Peak 5 appears to be a mixture of two isozymes; all but four matches are identical for subunits. Three of the matches are unique to GSTA1 and one is unique to GSTA2.