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SEC was performed by using a TosoHaas TSK G4000SW column as described in Materials and Methods. Squares, Mj HSP16.5–SCM at a 1:1 molar ratio, unheated; diamonds, Mj HSP16.5–SCM at a 1:1 molar ratio was heated at 80°C for 20 min and centrifuged at 10,000 × g for 10 min, and the supernatant was applied to the sizing column.
