Proteinase K susceptibility. Lane 1, molecular mass standards; lane 2,
SCM–Mj HSP16.5 at a 1:1 ratio, unheated; lane 3, SCM–Mj HSP16.5 at
a 1:1 ratio, heated at 80°C for 20 min and centrifuged (supernatant was
incubated with proteinase K as described in Materials and Methods);
lane 4, Mj HSP16.5 was heated at 80°C for 20 min and then treated with
proteinase K; lane 5, SCM was heated at 60°C for 20 min and then treated
with proteinase K.