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Phase-sensitive NOESY spectra of nornicotine protein glycation reactions. (A) Reaction of nornicotine, glucose-d7, and Aβ12–28. The cross-peaks observed between 2.59 and 2.68 ppm (marked with an arrow) are between the peptide and pyrrolidine resonances of nornicotine. (B) Reaction of nicotine, glucose-d7, and Aβ12–28. Critically, the cross-peaks observed with nornicotine are no longer present, whereas other internal nuclear Overhauser effects remain visible.
