Fig. 3.

Complementation of sensitivity of dna2-1 to MMS and HU. Exponentially growing yeast cultures of wild-type cells carrying empty vector (pRS316) and dna2-1 cells carrying empty vector, pGAL-hBLM, pGAL-hBLM(K695T), pGAL-FLAG-mWRN, pGAL-FLAG-RecQ4, and pGAL-hDNA2-FLAG were spotted in 10-fold serial dilutions onto SC galactose minus Ura plates with or without MMS or HU at the concentrations indicated and grown at the permissive temperature for 6 days. This experiment was performed in duplicate with identical results.