Fig. 2.

Eglin c residues with the potential to make adventitious contacts with Kex2/furin family enzymes. (Upper) Structure of eglin c alone, from crystal structure of a complex of eglin c with subtilisin Carlsberg. (Lower) Crystal structure of the complex (coordinates from 1cse.pdb, ref. 15). Structure was drawn with kinemage (28). Ten eglin c residues (exclusive of P₄-P′₁) whose side chains come within 7 Å of the subtilisin surface and that were mutagenized in this study are shown in green. Interacting enzyme residues are shown in gray and are indicated by residue numbers followed by E. P₁ Leu-45 of eglin c is shown in pink and catalytic His (His-64) is shown in purple.