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Rapid inhibition assay for novel eglin variants generated by randomization at position 49 in R4R1-eglin. (A) Furin inhibition by newly generated variants in two 96-well plates. The lower bar shows the level of inhibition by Tyr-49–R4R1-eglin. The upper bar indicates no inhibition. Glu (E), Asp (D), and Trp (W) substitutions are indicated by arrows. (B) Correlation plot of extent of inhibition of furin versus Kex2. (C) Correlation plot of extent of inhibition of furin versus PC7.
