Table 4. Effect of combining substitutions at positions 33 and 49 in R4R1-eglin.
Substitutions* | Kex2 | Furin | PC7 |
---|---|---|---|
Asp-33-Tyr-49 | 9.1 × 10-10† | 2.5 × 10-9 | 1.3 × 10-9 |
Asp-33-Asp-49 | 5.6 × 10-11 | 3.3 × 10-10 | 1.8 × 10-9 |
Val-33-Asp-49 | 4.2 × 10-11 | 2.7 × 10-10 | 1.1 × 10-8 |
All values were determined by using purified inhibitors as described in Materials and Methods. Experimental errors were ±20%
In the context of R4R1-eglin
Inhibition constants (Ki, M) determined by purified variants for Kex2, furin, and PC7