Fig. 2.

(A) Saturation isotherm and scatchard plot (Inset) of specific ¹²⁵I-Ang-(1–7) binding to Mas-transfected COS cells. Cells were incubated with increasing concentrations of ¹²⁵I-Ang-(1–7). No specific binding was determined in the presence of 1 μmol/liter Ang-(1–7). These data are represented as mean ± SEM of three different experiments. In the conditions used, the nonspecific binding averaged 40–60% of the total binding. (B) Competition for ¹²⁵I-Ang-(1–7) binding to Mas-transfected CHO cells by Ang-(1–7) and receptor antagonists. Competition curves were generated by adding increasing concentrations of CV-9174, PD 123319, A-779, and Ang-(1–7) to the incubation buffer containing 0.4 nmol/liter of ¹²⁵I-Ang-(1–7). Data are presented as mean ± SEM of three to six independent experiments.