. 2003 Jun 24;100(14):8308–8313. doi: 10.1073/pnas.1532535100

Table 1. Effects of ambiguous amino acids in TM I-III on the λ_max shift.

Pigment	Segment	Amino acid changes^*	λ_max, nm
a		WT	361 ± 1
	I	F₄₂60I₃₄	360 ± 1
	C-I	V₆₆63I₂₆	361 ± 1
	II	G₆₈82A₃₁/L₇₃85I₂₁/C₃₉87V₂₂/I₈₁88T₉/S₇₃92F₁₄/F₅₈96V₂₇/S₈₆98A₁₂/L₅₇99S₂₁/Q₆₃100R₁₇	362 ± 1
	E-I	V₄₇104I₃₅	361 ± 1
	III	H₅₇107R₃₁/V₈₂109L₈/L₈₄112M₁₃/L₅₀116M₄₉/V₆₈119I₂₈/I₆₆137V₃₃	361 ± 1
	C-II	K₆₄147R₃₆/G₄₉149S₄₅	361 ± 1
f		WT	393 ± 1
	I	V₅₀49L₃₅/I₇₁60V₂₉/V₅₉63I₄₁	392 ± 1
	II	F₈₁81V₁₃/I₅₁85M₂₀/I₇₅88V₂₅	392 ± 1
	III	A₇₁118S₁₈	398 ± 1
	C-II	N₈₇149S₁₃	392 ± 1

Subscripts show posterior probabilities of inferred amino acids

Table 1. Effects of ambiguous amino acids in TM I-III on the λmax shift.