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. 2000 Jun 27;97(14):7790–7795. doi: 10.1073/pnas.130201997

Figure 1.

Figure 1

Analysis of IscU expression and purification. (A) SDS/PAGE of overproduction and purification. Lane 1, molecular weight standards; lane 2, BL-21 cells harboring pTrcIscU (25 μg of protein); lane 3, BL-21 cells harboring pTrcIscU plasmid grown for ≈16 h after IPTG induction (25 μg of protein); lanes 4–6, purified IscU. (B) Immunoblot analysis of constitutive IscU expression in W3110, DH5α, and BL-21 cells grown to stationary phase in Terrific Broth. Lanes 1–3, purified IscU; lanes 4–6, E. coli whole cells (15 μg of protein each).