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Effects of IscU and Hsc20 on the ATPase activity of Hsc66. Time course of ATP hydrolysis at 23°C by 0.5 μM Hsc66 alone (○) or in the presence of 50 μM Hsc20 (■), 50 μM IscU (♦), or 50 μM IscU + 50 μM Hsc20 (▴). (Inset) An experiment with 5 μM Hsc66 and an expanded ordinate scale to show more clearly the activities in the presence of individual components.
