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. 2000 Jun 27;97(14):7790–7795. doi: 10.1073/pnas.130201997

Figure 4.

Figure 4

Effect of IscU–Fe/S on the ATPase activity of Hsc66. Results are reported as the increase in basal ATPase rates at 23°C. The curve shown for results in the presence of 50 μM Hsc20 represents a best fit to the data with maximal stimulation of 481-fold and half-maximal stimulation at 2.1 μM IscU–FeS; in the absence of Hsc20, maximal stimulation of 7.4-fold with half-maximal stimulation at 162 μM IscU. (Inset) The visible and near UV region absorption spectra for the metal-free form of IscU (apo-IscU, 81 μM) and IscU reconstituted with ferric iron and sulfide (IscU–Fe/S, 80 μM; see Experimental Procedures).