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. 2000 Jun 27;97(14):7790–7795. doi: 10.1073/pnas.130201997

Figure 5.

Figure 5

SPR analysis of the binding of IscU to Hsc66. IscU was injected into solutions passing over a sensor chip containing immobilized Hsc66 in the presence of 1 mM ATP (3,550 relative response units, RU) at 25°C, and maximal resonance signals observed are plotted as a function of the concentration of IscU. The equilibration solution contained HKM buffer, 1 mM ATP, and 5 mM DTT without (○) or with (●) 40 μM Hsc20 before injection of IscU. Experiments were carried out in duplicate, and error bars shown correspond to individual measurements when these fell outside the symbol used. The curves shown represent best fits to the data with RUmax = 206 and Kd = 8.9 μM in the presence of Hsc20 and RUmax = 193 and Kd = 39 μM in the absence of Hsc20. (Inset) Sensorgrams recorded after injections of 30 μM IscU in the absence or presence of 40 μM Hsc20.