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. 2000 Jun 27;97(14):7790–7795. doi: 10.1073/pnas.130201997

Figure 7.

Figure 7

Calorimetric analysis of the binding of IscU to Hsc20. A series of 42 equivalent 7-μl aliquots of 3.2 mM IscU were injected into a cell containing 1.348 ml of 0.19 mM Hsc20 at 25°C in HKM buffer containing 5 mM DTT. Integrated heats caused by binding, Qinj (corrected for the heats of dilution and divided by the moles of IscU injected), are plotted vs. the molar ratio of IscU and Hsc20 in the titration cell. The solid line represents a best-fit curve assuming 1.07 binding sites. Kd = 12.9 μM; ΔH = −3.53 kcal/mol; ΔS = 10.6 entropy units (e.u.).