Table 2. Flow cytometric analysis of selected T cell surface markers before and after two treatment cycles.

	HLA-DR+			CD45RO+			CD69+			CD4+CD25+			CD25+			CTLA-4+
Patient	Pre	Post	Pre	Post	Pre	Post	Pre	Post	Pre	Post	Pre	Post
	% of CD3+CD4+			% of CD3+CD4+			% of CD3+CD4+			% of CD3+			% of CD4+			% of CD4+CD25+
1	17.5	41.8	85.4	88.9	4.6	4.3	15.9	11.4	41.8	35.0	0.4	2.5
3	5.2	17.3	78.8	82.2	1.7	3.4	19.5	10.7	34.6	26.7	3.0	1.1
5	17.6	29.2	77.2	84.6	2.8	1.5	19.0	18.2	42.3	36.4	2.0	1.1
6	31.3	42.5	98.9	99.0	4.4	3.1	14.3	13.5	46.3	35.8	1.5	0.6
7	6.2	20.3	57.0	72.9	1.4	2.0	14.1	23.8	26.7	40.8	0.4	2.4
10	9.8	22.7	85.7	94.3	2.4	3.6	23.4	19.4	46.5	35.5	0.2	1.7
11	6.2	12.2	95.2	89.6	9.8	1.7	21.2	17.6	43.3	25.4	0.0	0.6
12	3.9	31.7	73.7	76.4	1.5	3.9	10.1	11.1	19.9	26.0	0.2	5.5
14	7.9	39.4	72.4	89.4	2.3	6.1	12.8	7.0	28.9	20.5	0.6	2.5
Mean Δ ± SEM	+16.8 ± 2.9 (P = 0.0004)			+5.9 ± 2.4 (P = 0.04)			-0.1 ± 1.1 (P = 0.9)			-1.9 ± 1.8 (P = 0.3)			-5.4 ± 3.2 (P = 0.13)			+1.1 ± 0.7 (P = 0.17)
	% of CD3+CD4-		%of CD3+CD4-		% of CD3+CD4-
1	30.5	57.9	66.1	69.0	7.7	6.8
3	14.8	22.3	52.4	58.8	3.6	3.9
5	28.9	31.3	53.8	55.8	4.3	2.7
6	78.0	67.4	51.3	61.4	12.5	8.2
7	12.3	13.3	52.9	43.7	6.7	2.5
10	29.6	44.3	80.2	82.0	2.1	7.8
11	4.9	18.2	77.6	70.4	8.1	7.4
12	8.4	35.8	69.6	72.1	3.8	8.9
14	14.1	20.7	57.9	60.9	7.0	7.6
Mean Δ ± SEM	+10.0 ± 4.1 (P = 0.04)			+1.4 ± 2.0 (P = 0.51)			0.0 ± 1.2 (P = 1.0)

Values compared by using paired t test