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. 2003 Jun 18;100(14):8384–8388. doi: 10.1073/pnas.0932778100

Fig. 2.

Fig. 2.

Multiple early insulitic T cell clones recognize IGRP206–214.58α-β- transfectants expressing the indicated TCRs were cultured with RMA-S/Kd cells pulsed with 1.0 (solid bars), 0.1 (open bars), or 0.01 μM (striped bars) of the indicated peptides, and IL-2 release was measured by ELISA. The partial TCRα and TCRβ chain sequences for 8.3 and for the early insulitic T cell clones AI12.B1.3 and AI15.F5 have been reported (3, 10, 13). The AI4, AI12.B1.1, AI12.B1.2, and AI15.A10 lines, all of which express non-Vα17 TCRα chains, did not respond to any of the peptides tested, although they were capable of signaling through the transfected TCR as evidenced by their release of IL-2 in response to plate-bound anti-CD3ε (data not shown). IGRP, IGRP206–214; INS, INS B15–23; FW, framework residues; CDR, complementarity-determining region.