NK cells in αGalCer-primed mice and IL-12Rβ2-/- mice
produce less IFN-γ.(A) Intracellular IFN-γ content of
DX5+TCRβ- cells from the liver 6 h after
αGalCer injection. Percentages of IFN-γ+ (boldface
line) or isotype+ cells are indicated. One representative mouse is
shown for each strain. For WT, n = 5; primed mice, n = 5;
and IL-12Rβ2-/- mice, n = 4. (B) Comparison
of IFN-γ levels in sera 6 h after αGalCer injection in WT,
asialo-GM1 treated, and IL-12Rβ2-/- mice. One representative
experiment of two is shown. Statistical analysis was performed by using
t tests to compare IFN-γ levels in WT versus GM1-treated and
IL-12Rβ2-/- mice. *, P = 0.003; **, P =
0.005.