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. 2003 Jun 25;100(14):8424–8429. doi: 10.1073/pnas.1431692100

Fig. 4.

Fig. 4.

Comparison of p53 transactivation function in yeast with that in human cells. (a) Transactivities of three representative p53 mutants (R175H, P177H, and M243V) through the indicated six p53-binding sequences were shown as relative GFP (EGFP or Ds-Red) intensities against wild-type (WT) p53 activities. (b) The three mutants, which were the same as a were examined for their abilities to transactivate the luciferase reporter gene through the six corresponding promoters in Saos-2 cells. Bar, SD. (c) Average mutation frequency on number of inactivated promoters. Based on the yeast experiment, the 2,314 mutants were fractionated on the number of inactivated promoters for eight distinct p53-binding sequences. (d) Average mutation frequency on number of inactivated promoters based on the transactivities of 89 selected mutants in Saos-2 cells. Note that we did not choose mutants that theoretically occur by C-to-T transition at CpG sites as well as the top 10 hot spot mutants to avoid strong bias. In both c and d, mutation frequency for each mutant was derived from the IARC database and was averaged in each fraction.