Table 1. Colony formation by spleen cells from 129/Sv recipients of Socs1-/- bone marrow transplants.
|
Mean no. of colonies
|
|||||||
|---|---|---|---|---|---|---|---|
| Marrow donor | Stimulus | Blast | G | GM | M | Eo | Meg |
| Socs1-/- (n = 10) | GM-CSF | 12±13 | 3±2 | 15±12 | 0.7±1.0 | ||
| IL-3 | 3±4 | 18±14 | 3±3 | 11±7 | 0.1±0.3 | 9±6 | |
| Socs1+/+ or Socs1+/- (n = 8) | GM-CSF | 1±1 | 0.5±0.5 | 1.8±2.4 | 0±0 | ||
| IL-3 | 0.3±0.7 | 1.1±1.4 | 0.5±0.5 | 2.9±2.1 | 0.1±0.4 | 1.1±1.0 | |
Data represent mean numbers of colonies ± SD developing in 7-day cultures of 50,000 spleen cells. Final concentrations of GM-CSF or IL-3 in the cultures were 10 ng/ml. Blast, blast colonies; G, granulocyte; GM, granulocyte/macrophage; M, macrophage; Eo, eosinophil; Meg, megakaryocyte colonies