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. 2003 Jun 26;100(14):8442–8447. doi: 10.1073/pnas.1430827100

Fig. 4.

Fig. 4.

Identification of the human Topo I residues responsible for enhancement of HIV-1 infectivity. (A) Schematic representation of the structures of Topo I mutants. Asterisks denote the six sites of African green monkey Topo I that differ from the human protein. White and shaded boxes indicate human and African green monkey Topo I, respectively. (B) Comparison of the infectivity of viruses derived from COS-1 cells expressing human WT, African green monkey WT, or mutant Topo I proteins. Data are means ± SD of three independent experiments. (C) Immunoblot analysis of COS-1 cells expressing WT or mutant Topo I with antibodies to the Xpress epitope tag. Lanes 1–8 correspond to the Topo I proteins indicated in A and B; lanes 9 and 10 correspond to cells transfected with a control vector (pcDNA) and to nontransfected cells, respectively.