Fig. 1.

Construction of an AML1-ETO-redirecting chimeric protein. (A) Model of antileukemic activity of a chimeric protein (M&M) consisting of the MYB DNA-binding domain and the MEF AML1-binding domain. (B) Construction of an expression vector for the chimeric M&M protein and a deletion mutant lacking DNA-binding activity. (C) Expression analysis of the proteins encoded by the various constructs. Immunoblotting of Cos cell lysates after transfection with GFP, GFP-ΔM&M, and GFP-M&M. Western blot analysis was performed by using an anti-GFP antibody.