Fig. 3.
GFP-M&M represses colony growth in cells expressing AML1-ETO. (A) 32D cells were transfected with GFP-pcDNA3.1 as a control or AML1-ETO and GFP-M&M as indicated, and a total of 1 × 105 cells were seeded in colony assays. Pictures of representative colonies were taken on day 10. (B) Transfected 32D cells (as in A) were seeded in colony assays. The repression of colony growth compared with the control transfection with GFP-pcDNA3.1 (set as 1) is shown. (C) Analysis of the importance of DNA binding of GFP-M&M for colony growth. AML1-ETO alone or in combination with either GFP-M&M or GFP-ΔM&M was transfected into 32D cells, which were subsequently plated in colony assays. (D) Human leukemic Kasumi-1 cells that naturally express AML1-ETO were transfected with GFP or GFP-M&M. Cells were subsequently plated in colony assays.