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Matrix-assisted laser desorption ionization mass spectra of the two oligodeoxynucleotide products resulting from cleavage of the 35mer DNA substrate. (A) The 5′ product, containing 16 nucleotides and a 3′-terminal phosphate, has an expected m/z for the principle product ion of 4,973. (B) The 3′ product, containing 18 nucleotides and a 5′-terminal phosphate, has an expected m/z of 5,646.
