Comparison of virus release from induced cells. (A) Time-course
analysis of virus release from induced human and mouse cell lines. HFF, SLK,
OT13, and OT11 were KSHV-infected for 2 h, followed by Ad-RTA superinfection.
Supernatants were collected every day and concentrated by ultracentrifugation.
The concentrated virus was then used to infect TIME cells, which were stained
for LANA at 48 h postinfection. Serial 2-fold dilutions of the concentrated
virus were used in the infectivity assay to ensure that the virus titer is in
linear range. The percentage of LANA-positive cells obtained with the
undiluted stock from each cell type is plotted against time (days
postinfection). ○, HEF; •, SLK; ▪, OT13; □, OT11.
(B) No infectious KSHV particles were released after RTA induction in
KSHV-infected OT11 cells. OT13 (Left)or OT11 (Right) cells
were infected with Ad-RTA alone (Top), KSHV alone (Middle),
or KSHV plus Ad-RTA (Bottom). Six days postinfection, supernatants
were collected from each culture, concentrated equivalently, and used to
infect HFF cells. Two days postinfection, HFFs were stained for LANA
expression by immunofluorescence assay.