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Results using pooled, convalescent sera after complete adsorption. (A) Individual, purified proteins were spotted on a nitrocellulose membrane and the membrane developed by using pooled convalescent sera. (B) Individual E. coli BL21 strains containing plasmids expressing specific proteins (or control plasmid, pET30a) were transferred to a nitrocellulose membrane and developed by using pooled patient sera after adsorption.
