Fig. 4.

TLR4 is necessary for LPS-induced neuronal injury in vitro. Mixed CNS cultures prepared from BALB/cJ and lps^d mouse forebrains were incubated with 10 μg/ml LPS alone or in combination with 20 mM KCl for 5 days. (A) Cultures were stained with antibody against neurofilament to mark axons and with DAPI. (Scale bar, 50 μm.) (B) Cultures were stained with antibody NeuN (neurons) and DAPI. (Scale bar, 50 μm.) (C) Quantitation of NeuN⁺ neurons from BALB/cJ and lps^d mice in the presence or absence of LPS. Experiments were repeated three times. Results are presented as mean ± SE. P < 0.001.