Effects of chronic treatment with EUK-189 or EUK-207 on lipid peroxidation
and protein oxidation in brain homogenates. At the end of the behavioral
experiments, the mice were killed and their brains (minus cerebellum) were
frozen. Levels of lipid peroxidation and protein oxidation (protein carbonyls)
were determined as described in Materials and Methods. Results were
expressed as percentage of vehicle control value (mean ± SEM,
n = 12; A and B). *, P < 0.05;
†, P < 0.01 (Student's t test). Levels of lipid
peroxidation and protein carbonyls were also determined in brain homogenates
from the 8-month-old control C57 mice. The levels of lipid peroxidation were
expressed as nmol malondialdehyde equivalent per mg of protein (C)
and the levels of protein oxidation as nmol carbonyl per mg of soluble
extracted protein (D), and are means ± SEM of 12 mice
[C: *, P < 0.05 as compared with either 8- or 11
month-old control mice; D: *, results not significantly different
from 8-month-old control mice; †, P < 0.005 as compared
with 11-month-old vehicle mice (Student's t test)].