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. 2003 Jun 18;100(14):8589–8594. doi: 10.1073/pnas.1430749100

Fig. 4.

Fig. 4.

Interaction between AtVAM3 and AtVTI11. (A) Extract from zig-1/pSCR::T7-ZIG plants was immunoprecipitated with anti-T7 monoclonal antibody (Left) or anti-AtVAM3 antiserum (Right), and then probed with the antibody or antisera indicated to the left. (B) Total extracts from wild-type (lane 1), sgr3-1/- homozygous (lane 2), and sgr3-1/+ heterozygous (lane 3) plants were probed with anti-AtVAM3 antiserum. (C) Interaction between mutant AtVAM3 protein and partner SNAREs. Extract from sgr3-1/+ zig-1/pSCR::T7-ZIG was immunoprecipitated with anti-T7 antibody or anti-SYP5 antiserum and then probed with anti-AtVAM3 antiserum. (D) A mixture of sgr3-1 zig-1/pSCR::T7-ZIG and zig-1 homozygous plant extracts was immunoprecipitated with anti-T7 antibody and then probed with anti-AtVAM3 antiserum. T, total protein extract; S, supernatant; IP, immunoprecipitate. The arrowhead and asterisk indicate wild-type and mutant AtVAM3 protein, respectively.