Table 2. Kinetic properties of cinnamic acid-activating At4CL2 variants.
|
Cinnamic acid
|
Caffeic acid
|
|||
|---|---|---|---|---|
| At4CL2 variant | Km, μM | Vmax, nkat/mg | Km, μM | Vmax, nkat/mg |
| WT | 6,642±972 | 203±23 | 22±5 | 236±30 |
| K320L | 1,010±135 | 150±19 | 62±11 | 188±27 |
| M293F + K320F | 930±83 | 113±33 | 361±188 | 90±20 |
| PL (M293P + K320L) | 286±13 | 190±44 | 41±8 | 267±91 |
| PL + N256A | 163±37 | 145±21 | 166±12 | 93±17 |