Figure 2.

IP/MS analysis of Aβ in the media of racemic, R- and S-flurbiprofen–treated cells. Plots shown are representative of two experiments, each performed in duplicate. CHO cells overexpressing APP751 and PS1ML were pretreated overnight with ethanol (a), 250 μM racemic flurbiprofen (b), 250 μM S-flurbiprofen (c), or 250 μM R-flurbiprofen (d). Conditioned medium was collected and analyzed after an additional 24 hours of treatment with the same concentration of the drug. Aβ peptides identified by their masses are indicated above the peaks. All forms of flurbiprofen reduce the Aβ42 peak and increase the levels of shorter Aβ derivatives to approximately the same extent. This effect is best seen by comparing the peak heights of the various peptides to the peak height of Aβ1–40, which is largely unchanged.