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. 2000 Jun 27;97(14):7829–7834. doi: 10.1073/pnas.130200197

Figure 1.

Figure 1

Estrogens suppress M-CSF/RANKL-induced osteoclast formation in primary murine myeloid cells. Nonadherent myeloid progenitors and monocytes were isolated from sham and ovariectomized mice and plated in triplicate at 105 cells/well. (A) Murine monocytes were treated with M-CSF (10 ng/ml) or M-CSF plus RANKL (30 ng/ml) for 10 days, stained for TRAP, and photographed at ×20. (B) Cells from sham (Left) or OVX (Right) mice were treated with M-CSF/RANKL in the presence of vehicle, 17β-estradiol (10−8 M), 17β-estradiol (10−8 M) plus ICI 182780 (10−6 M), 17α-estradiol (10−8 M), 4-hydroxytamoxifen (10−7 M), or raloxifene (10−7 M). Multinucleated (>3 nuclei), TRAP-positive cells were quantitated 10 days later. Numbers represent the mean ± SE, n = 3 (a is significant vs. b and c, and b is significant vs. c at P ≤ 0.05).