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. 2003 Aug 1;112(3):345–356. doi: 10.1172/JCI18698

Figure 1.

Figure 1

Foxa-2 is expressed in adipose tissue of obese mice. (a) Liver cell extracts from wild-type mice and adipocyte extracts from wild-type, ob/ob, db/db, and NZO mice were separated by SDS-PAGE and analyzed by Western blotting for Foxa-2 expression. TATA-binding protein (Tbp) expression was measured as a control for loading. (b) Visceral (v.) and subcutaneous (s.) fat RNA from wild-type and ob/ob mice was analyzed for Foxa-2 expression by Northern blotting. Membrane was rehybridized with a probe for cyclophilin as control. (c) Preadipocyte (Pre) and adipocyte (Ad) protein extracts from ob/ob mice were separated by SDS-PAGE and analyzed by Western blotting for Foxa-2 and aP2 expression. (d–f) Confocal image immunostaining of visceral fat from ob/ob mouse using anti–Foxa-2 Ab’s (d) and TO-PRO-3 (molecular probes) for nuclear staining (e). Images were superimposed (f). Foxa-2 protein is present in cytoplasm and nuclei. (g) Semiquantitative RT-PCR of Foxa-2 and Ucp-1 in white (WAT) and brown (BAT) adipose tissue of three ob/ob and three wild-type littermate mice. Hprt, hypoxanthine phosphoribosyltransferase.