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. 2003 Aug 1;112(3):345–356. doi: 10.1172/JCI18698

Figure 3.

Figure 3

Foxa-2 regulates the expression of Pref-1 and inhibits adipocyte differentiation. (a) Pref-1 expression is reduced in Foxa-2–deficient EBs. Wild-type (R1; +/+), heterozygous (B13, 4B1; +/–), and homozygous (B14, 5.1, 5.2; –/–) Foxa-2 ES cells were differentiated to EBs as described previously (27) and assayed for Hprt, Foxa-2, Gata-2, Gata-3, and Pref-1 mRNA expression by RT-PCR. –RT, reaction in absence of RT. (b) Anti–Pref-1 Ab’s render Foxa-2–overexpressing 3T3-L1 preadipocytes sensitive to differentiation. The 3T3-L1 cells that were transfected with empty vectors (pcDNA3), pCMV-Foxa-2 (Foxa-2), or pCMV-Pref-1 (Pref-1) were differentiated in the absence (Control) or presence of anti–Pref-1 Ab’s that were directed against the extracellular (Anti–Pref-1-N) or intracellular (Anti–Pref-1-C) domains.