Figure 8.

Altered insulin sensitivity in primary adipocytes of Foxa-2^+/– and wild-type littermates. Primary adipocytes were isolated from Foxa-2^+/– and wild-type littermate animals that had received a high-fat diet for 42 days. Parameters of glucose metabolism, lipogenesis and lipolysis were measured. (a–e) Glucose metabolism into different pathways at 10 and 100 nM insulin in isolated adipocytes from Foxa-2^+/– (gray) and wild-type (black) littermates. [U-¹⁴C]Glucose uptake in isolated adipocytes from epidydimal fat (a), [U-¹⁴C]glucose incorporation (incorp) into CO₂ (b), lactate (c), lipid glycerol (d), and lipid fatty acids (e), measured after 2 hours of incubation in the absence (control) or presence of insulin. (f) Glycerol release from adipocytes in the presence or absence of insulin (ins) after stimulation of lipolysis with isoperenterol (Isop). (g) Measurements of relative gene expression levels of metabolic genes in adipocytes of Foxa-2^+/– and wild-type littermates (100%) using semiquantitative RT-PCR. All mice were female, 15 weeks of age, n = 7, means ± SD. *P = 0.05; **P = 0.01; ***P = 0.001; ^#P = 10^–5.