Figure 1.

Association of HDAC4 and HDAC5 with two isoforms of 14-3-3. (A) Recombinant, FLAG-tagged HDAC1 and HDAC4 were transiently expressed in TAg Jurkat cells and were immunoprecipitated by using α-FLAG agarose (Sigma). The immunopurified complexes were separated by SDS/PAGE, and the proteins were visualized by silver stain. (B) The association between HDAC4 and HDAC5 with 14-3-3 was confirmed by Western blot analysis. The recombinant FLAG-tagged proteins were subjected to Western blot analysis using 14-3-3 isoform specific antibodies.